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Merck

42395

Sigma-Aldrich

Tannase aus Aspergillus ficuum

powder, white, ≥150 U/g

Synonym(e):

Tannin Acyl-Hydrolase

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About This Item

CAS-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

Aspergillus sp. (A. ficuum)

Qualitätsniveau

Form

powder

Spezifische Aktivität

≥150 U/g

Verunreinigungen

25 mM potassium phosphate
250 mM NaCl
50% glycerol

Farbe

white

Lagertemp.

2-8°C

Allgemeine Beschreibung

Tannase is an enzyme that is produced by several organisms such as plants, bacteria, fungi, and yeast. This enzyme is also found in plant sources such as tannin-rich vegetables, especially in the leaves, fruits, branches, and bark.

Anwendung

Tannase from Aspergillus ficuum has been used:
  • as a standard to determine the tannase activity of bacterial isolates
  • to study its effects on the inhibitory activity of tannic acid on biofilm formation
  • to obtain Proanthocyanidins (PA) by enzymatic hydrolysis of grape skin and seeds

Biochem./physiol. Wirkung

Tannase catalyzes the ester bonds located in complex tannins, gallo-tannins, and gallic acid esters, which results in the release of gallic acid. This enzyme can be used as a clarifying agent in the manufacture of beer, tea, wine, and juices and to treat tannin-polluting agricultural waste and industrial effluents.

Einheitendefinition

1 U entspricht der Enzymmenge, die bei 310 nm eine Extinktionsänderung von 1.0 pro Minute bei pH 4.7 und 30°C bewirkt (Tannin als Substrat, 3 mL Endvolumen)

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Arijit Jana et al.
Applied biochemistry and biotechnology, 167(5), 1254-1269 (2012-01-25)
Tannase production by newly isolated Penicillium purpurogenum PAF6 was investigated by 'one variable at a time' (OVAT) approach followed by response surface methodology (RSM). Tannin-rich plant residues were used as supporting solid substrate and sole carbon source and, among them
Jose Valdo Madeira et al.
Bioprocess and biosystems engineering, 35(3), 477-482 (2011-09-13)
The production of enzymes such as tannases and phytases by solid-state fermentation and their use in animal feed have become a subject of great interest. In the present work, Paecilomyces variotii was used to produce tannase and phytase simultaneously. Solid-state
Jose Valdo Madeira et al.
Bioresource technology, 102(15), 7343-7348 (2011-05-27)
In this work, we introduce a biological detoxification method that converts toxic waste from castor beans into animal feed material. This method simultaneously induces the production of tannase and phytase by Paecilomyces variotii; both enzymes have high levels of activity
Bhakti Bajpai et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 39(4), 708-711 (2008-10-01)
In a new approach to microbial gallic acid production by Aspergillus fischeri MTCC 150, 40gL(-1) of tannic acid was added in two installments during the bioconversion phase of the process (25gL(-1) and 15gL(-1) at 32 and 44h respectively). The optimum
Tadashi Takahashi et al.
Eukaryotic cell, 11(4), 507-517 (2012-01-31)
Loop-out-type recombination is a type of intrachromosomal recombination followed by the excision of a chromosomal region. The detailed mechanism underlying this recombination and the genes involved in loop-out recombination remain unknown. In the present study, we investigated the functions of

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