10021101

SerBob cell line human

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human prostate
• Qualitätsniveau: 100
• Form: liquid
• Wachstumsmodus: Adherent
• Karyotyp: 4n (67 to 88)
• Morphologie: Cobblestone
• Produkte: More differentiated than Bob with increased CD24, PAP, PSCA expression (prostate differentiation markers). 10% 3-D sphere formation in Matrigel. Migratory and invasive when compared to benign prostate tumour. More invasive than Bob.
• Rezeptoren: CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human prostate cancer spontaneously immortalized

Beschreibung der Zelllinie

First spontaneously immortalized prostate cancer cell line to be established from a trans-rectal needle biopsy (TRBP) of a patient with castration-resistant prostate cancer (CRPC). Bob is a novel pre-clinical model for functional studies in CRPC and especially for studying the CRPC "basal" phenotype. SerBob grows as a monolayer culture with limited differentiation. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Anwendung

Prostate cancer research including drug testing.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,14
D16S539: 10,12
D5S818: 13
D7S820: 10,11
THO1: 9
TPOX: 8,10
vWA: 17

Nährmedium

Keratinocyte-SFM (Invitrogen, catalogue no 37010-022, supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor + 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. At confluence 5-7 x 104 cells/cm² can be expected.

Sonstige Hinweise

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Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable