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Merck

C-14090

Sigma-Aldrich

Human Mesenchymal Stem Cells (hMSC)

Derived from bone marrow, 1 million cells per pellet

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About This Item

UNSPSC-Code:
41106514

Biologische Quelle

human bone marrow

Verpackung

pkg of 1,000,000 cells

Morphologie

(stromal)

Anwendung(en)

cell analysis (DNA, RNA, and protein)

Versandbedingung

dry ice

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Ursprung der Zelllinie

Bone Marrow

Anwendung

Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized PromoCell media are available to support both the growth of MSC and their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into diverse cell types vary between MSC of different origin.Human Mesenchymal Stem Cells (hMSC-BM) are harvested from normal human bone marrow of individual donors. The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts. The cells show a verified marker expression profile that complies with ISCT recommendations, providing well characterized cells (Cytotherapy (2006) Vol. 8, No. 4, 315-317).

Qualität

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warnhinweis

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Physikalische Form

Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Subkultur-Routine

Click here for more information.

Sonstige Hinweise

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Rechtliche Hinweise

RNAlater is a registered trademark of Ambion, Inc.

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Zulassungslistungen

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EU REACH Annex XVII (Restriction List)

CAS No.

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and product use.

Protocol to differentiate human mesenchymal stem cells into neurons with extensive dendrites and axons in 3 days. Explore over 350 PromoCell products on Sigma.com.

Are your mesenchymal stem cells really MSCs? Learn more about the MSC characterization guidelines proposed by the International Society for Cellular Therapy (ISCT).

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

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Protokolle

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and product use.

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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