AT-1

94101449, rat prostate, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: AT-1, 94101449
• Biologische Quelle: rat prostate
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 62
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat Dunning R-3327,dorsal prostatic adenocarcinoma

Beschreibung der Zelllinie

Derived from a spontaneous Dunning R-3327 rat prostatic cancer. The cells have been shown to be anaplastic with low metastatic ability and non-responsive to androgen.

Nährmedium

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 2-4 x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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