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ROAMYGLL

Roche

Amyloglucosidase

from Aspergillus niger

Synonym(e):

Aspergillus niger, disaccharidase-type a-glucosidase

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About This Item

EC-Nummer:
3.2.1.3 (BRENDA, IUBMB)
UNSPSC-Code:
12352204

Biologische Quelle

Aspergillus niger

Qualitätsniveau

100

Form

lyophilized

Spezifische Aktivität

6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate)
~6 units/mg protein (At 25 °C with glycogen as the substrate.)

Mol-Gew.

97 kDa

Verpackung

pkg of 3,500 U (11202367001)
pkg of 500 U (11202332001)

Hersteller/Markenname

Roche

Optimaler pH-Wert

4.6-4.8

Lagertemp.

2-8°C

Allgemeine Beschreibung

Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase. It is synthesized by several Aspergillus genus species. This exo-enzyme is one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.

Spezifität

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.

Anwendung

Amyloglucosidase from Aspergillus niger is used for the determination of starch.

Biochem./physiol. Wirkung

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.

Angaben zur Herstellung

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:
  • Western blot: 1 to 10 μg/ml


Working solution: Tris-buffered saline containing 0.1% Tween 20.

Storage conditions (working solution): 2 to 8 °C

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

does not flash

Flammpunkt (°C)

does not flash

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

High fructose corn syrup: production, uses and public health concerns.
Parker K, et al.
Biotechnology and Molecular Biology Reviews, 5, 71-78 (2010)
Simultaneous amyloglucosidase and exo-polygalacturonase production by Aspergillus niger using solid-state fermentation.
Costa, J et al.
Brazilian Archives of Biology and Technology, 50, 759-766 (2007)
High-potency amyloglucosidase-producing mold of the Aspergillus niger group.
Smiley K L, et al.
Applied Microbiology, 12(5), 455-455 (1964)
Nicholson N.
Biodiversity: New Leads for the Pharmaceutical and Agrochemical Industries (2000)
Juliann R Seebauer et al.
Journal of experimental botany, 61(2), 511-519 (2009-11-18)
The relative role of the maternal source and the filial sink in controlling the composition of maize (Zea mays L.) kernels is unclear and may be influenced by the genotype and the N supply. The objective of this study was
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