QIA39

FragEL DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme

• Synonym(e): TUNEL Assay
• UNSPSC-Code: 41116133
• NACRES: NA.51

Eigenschaften

• Verwendung: sufficient for 50 tests
• Qualitätsniveau: 100
• Speziesreaktivität (Voraussage durch Homologie): all
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Methode(n): flow cytometry: suitable
• Aufnahme: sample type cell suspension(s); sample type frozen section(s); sample type paraffin section(s); sample type fixed-cell preparation(s)
• Nachweisverfahren: fluorometric
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Allows for detection of apoptosis at the individual cell level. Apoptosis morphology is easily detected. Detection is by flow cytometry or fluorescence microscopy. The kit mounting media permits the observance of the total cell population.

Cat. No. QIA39 is a fluorescein-conjugated version of our widely referenced TdT Colorimetric FragEL DNA Fragmentation Detection Kit (Cat. No. QIA33) for non-isotopic labeling. Terminal deoxynucleotidyl transferase (TdT) binds to exposed 3′-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of fluorescein-labeled and unlabeled deoxynucleotides. When excited, fluorescein generates an intense signal which can be detected by fluorescence microscopy or flow cytometry. The mounting media sustains the fluorescent signal from samples labeled on slides and, therefore, aids in the morphological evaluation and characterization of normal and apoptotic cells. Non-apoptotic cells can be visualized by a DAPI filter.

Komponenten

Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Control Slides, Mounting Media (Cat. No. HC08), and a user protocol.

Warnhinweis

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Spezifikationen

Assay Time: 2 h

Prinzip

The Calbiochem Fluorescein-FragEL DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, in cell preparations fixed on slides, or in cell suspensions. Detection and analysis of labeling is possible by flow cytometry or fluorescence microscopy.

Lagerung und Haltbarkeit

Fluorescein-FragEL kit components are shipped on dry ice. Upon receipt, store kit at -20°C in a non-frost-free freezer. To avoid reagent loss in tube caps, briefly pulse spin all thawed solutions before removing caps. (see General Considerations below).

Sonstige Hinweise

Darzynkiewicz, Z., et al. 1997. Cytometry27, 1.
Frey, T. 1997. Cytometry28, 253.
Shapiro, H.M. 1995. Practical Flow Cytometry, Third Edition. Wiley-Liss Inc., New York, New York.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
Gavrieli, Y., et al. 1992. J. Cell Biol.119, 493.
Fawthrop, D. J., et al. 1991. Arch. Toxicol.65, 437.
Martin, S.J., et al. 1990. J. Immunology145, 1859.
Wyllie, A. H. 1980. Nature284, 555.
Kerr, J. F. R., et al. 1972. Br. J. Cancer26, 239.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Parafilm is a registered trademark of Bemis Company, Inc.

Sicherheitshinweise

• Piktogramme: GHS08,GHS07,GHS09
• Signalwort: Danger
• H-Sätze: H302 + H332,H319,H350,H411
• P-Sätze: P202 - P273 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Gefahreneinstufungen: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B - Eye Irrit. 2
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects