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CY0100

Sigma-Aldrich

Cytochrome c Reductase (NADPH) Assay Kit

1 kit sufficient for 100 tests, determining cytochrome c reductase activity

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Qualitätsniveau

200

Verwendung

 kit sufficient for 100 tests

Versandbedingung

dry ice

Lagertemp.

−20°C

Angaben zum Gen

human ... POR(5447)

Verwandte Kategorien

Biochem./physiol. Wirkung

Eukaroytic NADPH-cytochrome c reductase (NADPH-cytochrome P450 reductase, EC1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which are the cytochrome P450 family of enzymes, responsible for xenobiotic detoxification. NADPH-cytochrome c reductase is widely used as an endoplasmic reticulum marker and as one of the biomarkers for ecological pollution and dietary lipid uptake.

Leistungsmerkmale und Vorteile

  • Detection of endoplasmic reticulum (ER) during isolation and subcellular fractionation by density gradient separation
  • Colorimetric measurement of NADPH-Cytochrome C Reductase activity in various sources after intoxication by drugs or challenge by xenobiotics
  • Analyze enrichment and function of ER

Hinweis zur Analyse

The Cytochrome C Reductase Assay Kit contains all the reagents required for a fast and convenient determination of NADPH-Cytochrome C Reductase activity in cell and tissue extracts and in purified microsomes. It has been tested on samples prepared from various species of mammalian tissues such as liver, kidney, brain, spleen, and heart muscle, as well as on lysates from cell lines such as HeLa, HepG2, and Jurkat. In addition, it has been tested on samples prepared from the yeast strains P. pastoris and S. cerevisiae.

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • Assay Buffer 100 mL
  • Enzyme Dilution Buffer 25 mL
  • Cytochrome c 50 mg
  • Cytochrome c Reductase (NADPH) Positive Control from rabbit liver 50 μL
  • Cytochrome c Oxidase Inhibitor Solution 1 mL

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB
  • N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mgSDB

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Piktogramme

Skull and crossbones
GHS06

Signalwort

Danger

H-Sätze

H301,H412

Gefahreneinstufungen

Acute Tox. 3 Oral - Aquatic Chronic 3

Lagerklassenschlüssel

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Rohit Sharma et al.
Age (Dordrecht, Netherlands), 36(4), 9686-9686 (2014-07-20)
Imbalance in Th1/Th2 immune pathways and cellular antioxidant systems with progressive aging are among the leading causes of increased risk of morbidity and mortality in elderly. Although probiotics have been considered to boost immune system, there is a lack of
Nathan M Long et al.
Reproductive biology and endocrinology : RB&E, 11, 34-34 (2013-05-10)
Previously we reported decreased circulating progesterone and fertility in one and two year old ewes born to undernourished mothers. This study was designed to investigate if this reduction in progesterone persisted into old age, and if it did, what mechanisms
D Plonne et al.
Analytical biochemistry, 276(1), 88-96 (1999-12-10)
A novel method is described for the separation on a single gradient of the major intracellular organelles of the secretory pathway, the Golgi, the smooth endoplasmic reticulum, and the rough endoplasmic reticulum. Total microsomes were prepared from rat liver by
Amanda Spring de Almeida et al.
Life sciences, 276, 119469-119469 (2021-04-04)
Breast cancer-induced chronic pain is usually treated with opioids, but these compounds cause various adverse effects. Transient receptor potential ankyrin 1 (TRPA1) is involved in cancer pain; also, endogenous TRPA1 agonists are associated with cancer pain development. The aim of
C O Lemley et al.
Journal of dairy science, 93(3), 1012-1021 (2010-02-23)
In the cow, inadequate concentrations of progesterone during gestation may lead to an abrupt termination of pregnancy. The primary organ involved in progesterone catabolism is the liver, which contains an abundance of cytochrome P450 isozymes (EC 1.14.14.1; mixed-function monooxygenases). The
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