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Key Documents

C1427

Sigma-Aldrich

Cytochrome b5 human

recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)

Synonym(e):

Cytochrome b5

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About This Item

MDL-Nummer:
MFCD02687283
UNSPSC-Code:
12161501
NACRES:
NA.47

Biologische Quelle

human

Qualitätsniveau

200

Rekombinant

expressed in E. coli (histidine-tagged)

Assay

≥90% (SDS-PAGE)

Form

solution

Mol-Gew.

16 kDa

Methode(n)

inhibition assay: suitable

Eignung

suitable for molecular biology

UniProt-Hinterlegungsnummer

P00167

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... CYB5A(1528)

Allgemeine Beschreibung

Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.

Biochem./physiol. Wirkung

Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.
Cytochrome b5 is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.

Physikalische Form

Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA

Hinweis zur Analyse

Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b5.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

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Sigma-Aldrich

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Sigma-Aldrich

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Sigma-Aldrich

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Melissa R Pitman et al.
Scientific reports, 12(1), 454-454 (2022-01-12)
Sphingosine 1-phosphate (S1P) is a signaling lipid that has broad roles, working either intracellularly through various protein targets, or extracellularly via a family of five G-protein coupled receptors. Agents that selectively and specifically target each of the S1P receptors have
Human adrenal cells that express both beta-hydroxysteroid dehydrogenase type 2 (HSD3B2) and cytochrome b5 (CYB5A) contribute to adrenal androstenedione production
Nakamura Y, et al.
The Journal of Steroid Biochemistry and Molecular Biology, 123(3-5), 122-126 (2011)
CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women
Stark K, et al.
Arthritis Research & Therapy, 17(1), 56-56 (2015)

Artikel

Electron Transport

Learn about the four membrane-bound protein complexes that make up the electron transport chain metabolic pathway supplying energy as ATP for cellular respiration.

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