ABE1455

Anti-Nurr1 (NR4A2)

from rabbit, purified by affinity chromatography

• Synonym(e): Nuclear receptor subfamily 4 group A member 2, Immediate-early response protein NOT, Orphan nuclear receptor NURR1, Transcriptionally-inducible nuclear receptor
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• Verpackung: antibody small pack of 25 μg
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_006177.1
• UniProt-Hinterlegungsnummer: P43354
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NR4A2(4929)

Beschreibung

Allgemeine Beschreibung

Nuclear receptor subfamily 4 group A member 2 (UniProt: P43354; also known as Immediate-early response protein NOT, Orphan nuclear receptor NURR1, Transcriptionally-inducible nuclear receptor) and is encoded by the NR4A2 (also known as NOT, NURR1, TINUR) gene (Gene ID: 4929) in human. Nurr-1 belongs to the nuclear hormone receptor family that acts as a transcriptional regulator and is considered to be important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is also crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. It is expressed in a number of T cell, B cell and fibroblast cell lines, but its expression is higher in brain tissue. Two isoforms of Nurr-1 have been reported that are produced by alternative splicing. The ligand-binding domain (LBD) of Nurr-1 contains no cavity as a result of the tight packing of side chains from several bulky hydrophobic residues in the region normally occupied by ligands. It also lacks a classical binding site for co-activators. However, it has hydrophobic region on the surface of LBD, which is critical for its activity. (Ref.: Volakakis N et al (2006). J. Mol. Endocrinol 37(2); 317-326; Wang G et al. (2003). Nature 423(6939):555-560).

Spezifität

This rabbit polyclonal antibody detects NR4A2 (Nurr1) in human cells. It targets an epitope within 16 amino acids from the N-terminal region.

Immunogen

Epitope: N-terminus

KLH-conjugated liner peptide corresponding to 16 amino acids from the N-terminal region of human Nuclear receptor subfamily 4 group A member 2 (Nurr1).

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Anti-Nurr1 (NR4A2), Cat. No. ABE1455, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 4 group A member 2 and has been tested for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Nurr1 (NR4A2) in HeLa cells.

Qualität

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Nurr1 (NR4A2) in THP-1 cell lysate.

Zielbeschreibung

~67 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.