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Merck

1.46208

Millipore

ReadyTube® Fraser Broth

For enrichment of samples for Listeria sp. and Listeria monocytogenes in accordance to ISO11290, pkg of (10 mL in 17 mL tube with blue cap (20 or 100 tubes per box))

Synonym(e):

Fraser Broth, Full Fraser Broth

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About This Item

UNSPSC-Code:
41106225

Agentur

ISO 11290

Form

liquid (ready-to-use)

Leistungsmerkmale

ready-to-use

Hersteller/Markenname

ReadyTube®

Verpackung

pkg of (10 mL in 17 mL tube with blue cap (20 or 100 tubes per box))

pH-Wert

7.2 ( in H2O)

Anwendung(en)

environmental
food and beverages
microbiology

Lagertemp.

2-8°C

Eignung

Listeria spp.

Verwandte Kategorien

Allgemeine Beschreibung

ReadyTube® is ready to use media to provide the highest level of quality and testing confidence for improving accuracy and significantly reducing the risk of false results. ReadyTube® Fraser broth is a selective enrichment medium designed to help the recovery of Listeria sp.

Anwendung

Application: Enrichment of Listeria spp.

Packaging: 10 ml in 17 ml tube with blue cap (20 or 100 tubes per box)

Synonym: Fraser Broth, Full Fraser Broth

Hinweis zur Analyse

This medium contains peptone, casein, yeast extract, and meat extract, which provide essential nutrients like carbon and nitrogenous compounds, including vitamins, amino acids, and trace ingredients. Phosphates buffer the medium while sodium chloride maintains osmotic equilibrium. Nalidixic acid and acriflavine inhibit the growth of Gram-negative and Gram-positive organisms respectively, except Listeria sp. Listeria sp. hydrolyze esculin to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate, resulting in the formation of 6-7-Dihydroxycoumarin, a black-brown complex. Ferric ammonium citrate also enhances the growth of L. monocytogenes. The high salt tolerance (of sodium chloride) of Listeria is used as means to inhibit the growth of Enterococci. Lithium chloride is also used to inhibit Enterococci, which also possess the ability to hydrolyze esculin.

Rechtliche Hinweise

READYTUBE is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2


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Artikel

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

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