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LC/MS/MS Analysis of Drugs of Abuse: Generic Screening on Ascentis® Express F5

LC/MS/MS Analysis of Drugs of Abuse: Generic Screening on Ascentis® Express F5 application for HPLC

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

53569-U

Ascentis® Express F5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

Standard

N° Catalogo
Descrizione
Determinazione del prezzo

B-004

Benzoylecgonine solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

C-006

Codeine solution

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

F-013

Fentanyl solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

H-003

Hydrocodone solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-005

Morphine solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-009

(±)-Methamphetamine solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-013

(±)-MDMA solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-035

Meperidine solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

N-017

Normeperidine solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

O-002

Oxycodone solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

P-011

(+)-Propoxyphene solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

mobile phase

[A] 5 mM ammonium formate water pH 4.0 formic acid; [B] 5 mM ammonium formate (95:5 acetonitrile:water)

gradient

20% B for 0.5 min; to % B in 5.5 min; held at 100% B for 3 min

flow rate

0.3 mL/min

pressure

2538 psi (175 bar)

column temp.

35 °C

detector

MS, ESI(+), TIC, m/z 100-1000

injection

1 μL

sample

each compound 1000 ng/mL in 40:60 (water:methanol)

Descrizione

Risultati analitici

Shown here is a method to rapidly screen a sample for a variety of abused substances using an Ascentis Expres F5 column. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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