HPLC Analysis of Ephedrine Compounds on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)

mobile phase

[A] acetonitrile; [B] 40 mM ammonium acetate in water; (90:10, A:B)

flow rate

0.4 mL

column temp.

35 °C

detector

UV, 254 nm

injection

1 μL

sample

150 μg/mL each in mobile phase