HPLC Analysis of Bile Acids on Ascentis^® Express C18

column

Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 μm particles (53826-U)

mobile phase

[A] 10 mM ammonium acetate, pH 4 (adjusted with acetic acid); [B] acetonitrile; (80:20, A:B)

gradient

20 to 100% B in 10 min

flow rate

1.0 mL/min

column temp.

35 °C

detector

ESI(-) in SIR mode

injection

5 μL

sample

10 μg/mL