Passa al contenuto

Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Organic & biomolecular chemistry (2015-01-03)

Sevnur Serim, Philipp Baer, Steven H L Verhelst

ABSTRACT

Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for serine proteases based on a phosphonate ester scaffold.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

Sigma-Aldrich

Acido acetico, glacial, ACS reagent, ≥99.7%

Sigma-Aldrich

Acido acetico, glacial, ReagentPlus^®, ≥99%

Sigma-Aldrich

Solfato di magnesio, anhydrous, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

2-butanone, ACS reagent, ≥99.0%

Sigma-Aldrich

Acido acetico, glacial, ≥99.99% trace metals basis

Sigma-Aldrich

Acido acetico, suitable for HPLC

Sigma-Aldrich

(+)- L-ascorbato di sodio, crystalline, ≥98%

Sigma-Aldrich

Acido acetico, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%

Sigma-Aldrich

Acido acetico, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%

Sigma-Aldrich

2-butanone, suitable for HPLC, ≥99.7%

Sigma-Aldrich

Bromuro di litio, ReagentPlus^®, ≥99%

Sigma-Aldrich

Solfato di magnesio, anhydrous, reagent grade, ≥97%

Sigma-Aldrich

Di-tert-butyl dicarbonate, ReagentPlus^®, 99%

Sigma-Aldrich

HATU, 97%

Sigma-Aldrich

(+)- L-ascorbato di sodio, powder, BioReagent, suitable for cell culture

Sigma-Aldrich

(+)- L-ascorbato di sodio, BioXtra, ≥99.0% (NT)

Sigma-Aldrich

Solfato di magnesio, BioReagent, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

Solfato di magnesio, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

Copper(I) bromide, 98%

Sigma-Aldrich

Acido acetico, for luminescence, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Bromuro di litio, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99%

Sigma-Aldrich

Solfato di magnesio, puriss. p.a., drying agent, anhydrous, ≥98.0% (KT), powder (very fine)