ZRB2282

Anti-DNAJB9 Antibody, clone 1D16-K ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinonimo/i: ER-resident protein ERdj4, Endoplasmic reticulum DNA J domain-containing protein 4, Microvascular endothelial differentiation gene 1 protein, UNQ743/PRO1471
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 200
• Ricombinante: expressed in HEK 293 cells
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 1D16-K, recombinant monoclonal
• Descrizione: recombinant, expressed in HEK 293 cells
• Nome Commerciale: ZooMAb^® learn more
• Stato: lyophilized
• PM: calculated mol wt 25.52 kDa; observed mol wt ~25 kDa
• Purificato mediante: using Protein A
• Reattività contro le specie: human, mouse, rat
• Reattività contro le specie (prevista in base all’omologia): monkey
• Confezionamento: antibody small pack of 25
• Caratteristiche più verdi: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Convalida avanzata: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• tecniche: affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG
• Sequenza dell’epitopo: Internal
• N° accesso ID proteina: NP_036460
• N° accesso UniProt: Q9UBS3
• Categoria alternativa più verde: Aligned
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: human ... DNAJB9(4189)

Descrizione

Descrizione generale

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificità

Clone 1D16-K is a ZooMAb^® rabbit recombinant monoclonal antibody that detects DNAJB9. It targets an epitope within 23 amino acids from the C-terminal half of the protein.

Immunogeno

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal half of human DNAJB9.

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DNAJB9 in mouse kidney tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected DNAJB9 in rat kidney tissue lysates.

Affinity Binding Assay: The binding affinity of this antibody to DNAJB9 peptide was at least one hundred thousand-fold (100,000X) higher than that obtained with the non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DNAJB9 in human kidney and mouse kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrizione del bersaglio

DnaJ homolog subfamily B member 9 (UniProt: Q9UBS3; also known as Endoplasmic reticulum DNA J domain-containing protein 4, ER-resident protein ERdj4, Microvascular endothelial differentiation gene 1 protein) is encoded by the DNAJB9 (also known as MDG1, UNQ743/PRO1471) gene (Gene ID:4189) in human. DNAJB9 belongs to the DnaJ/Hsp40 protein family, which is involved in chaperone-mediated protein folding and cellular stress responses. DNAJB9 consists of various functional domains, including a J domain, which is responsible for interacting with Hsp70 chaperones, and a cysteine-rich domain implicated in protein-protein interactions. The expression profile of DNAJB9 varies across different tissues and cell types. It is expressed in various organs, including the brain, heart, liver, and kidney. DNAJB9 expression levels can be regulated under different physiological and pathological conditions, such as during cellular stress or in certain disease states. Increased expression of DNAJB9 has been observed in certain brain regions affected by diseases. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Stato fisico

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Ricostituzione

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Note legali

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable