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Key Documents

ZRB1448

Sigma-Aldrich

Anti-Recoverin Antibody, clone 1K10 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinonimo/i:

Cancer-associated retinopathy antigen, Cancer-associated retinopathy protein, Protein CAR

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

rabbit (recombinant)

Livello qualitativo

Ricombinante

expressed in HEK 293 cells

Coniugato

unconjugated

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

1K10, monoclonal
recombinant monoclonal

Nome Commerciale

ZooMAb® learn more

Forma fisica

lyophilized

PM

calculated mol wt 23.13 kDa
observed mol wt 20 kDa

Reattività contro le specie

human, bovine

Confezionamento

antibody small pack of 25 μL

Caratteristiche più verdi

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Convalida avanzata

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

tecniche

affinity binding assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

Isotipo

IgG

N° accesso UniProt

Categoria alternativa più verde

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... RCVRN(5957)

Descrizione generale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificità

Clone 1K10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Recoverin.

Immunogeno

His-tagged full-length human recombinant Recoverin.

Applicazioni

Anti-Recoverin, clone 1K10 ZooMAb, Cat. No. ZRB1448, is a recombinant rabbit monoclonal antibody that specifically targets Recoverin and is tested for use in Affinity Binding Assay, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Recoverin in human retina tissue sections.

Immunofluorescence Analysis: 1:500 dilution from a representative lot detected Recoverin in human retina tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant recoverin protein with a KD of 6.0 x 10-9 in an affinity binding assay.

Immunocytochemistry Analysis: 1:100 dilution of this antibody detected Recoverin in Y79 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Descrizione del bersaglio

Recoverin (UniProt P35243; also known as Cancer-associated retinopathy antigen, Cancer-associated retinopathy protein, Protein CAR) is encoded by the RCVRN (also known as RCV1) gene (Gene ID 5957) in human. The photoreceptor protein recoverin is a member of the EF-hand neuronal calcium sensor (NCS) proteins. It acts as a calcium sensor and regulates phototransduction of cone and rod photoreceptor cells. It modulates light sensitivity of cone photoreceptor in dark and dim conditions. It improves rod photoreceptor sensitivity in dim light and mediates response of rod photoreceptors to facilitate detection of change and motion in bright light. In response to high Ca2+ levels induced by low light levels, it prolongs RHO/rhodopsin activation in rod photoreceptor cells by binding to and inhibiting G-protein-coupled receptor kinase 1 (GRK1)-mediated phosphorylation of RHO/rhodopsin. Recoverin is composed of four EF-hand domains (aa 25-60, 61-96, 97-132, 147-182), with the second and third EF-hand regions mediating low- and high-affinity calcium binding, respectively. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Stato fisico

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Ricostituzione

0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Note legali

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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