Y0396

Anti-YB-1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-BP-8, Anti-CSDA2, Anti-CSDB, Anti-DBPB, Anti-MDR-NF1, Anti-NSEP1, Anti-Y BOX binding protein, Anti-YBOX1
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: buffered aqueous solution
• PM: antigen ~50 kDa
• Reattività contro le specie: rat, human, mouse
• Concentrazione: ~1.0 mg/mL
• tecniche: immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates; indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells; western blot: 0.5-1 μg/mL using PC12 cell lysates
• N° accesso UniProt: P67809
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... YBX1(4904) ; mouse ... Ybx1(22608) ; rat ... Ybx1(500538)

Descrizione

Descrizione generale

Y-box-binding protein (YB-1) also known as nuclease-sensitive element-binding protein 1 (NSEP1) is a member of the Cold-Sock Domain (CSD) containing proteins. It is composed of three domains: the N-terminal which is thought to function as a trans-activation regulatory domain, a highly conserved nucleic acid binding domain (the CSD), and C-terminal domain, involved in protein-protein interactions.

Specificità

Anti-YB-1 (C-terminal) antibody is specific for YB-1 in rats, mice and humans. The immunizing peptide specifically inhibits the detection of the YB-1 band by immunoblotting.

Immunogeno

synthetic peptide corresponding to amino acids 307-324 of human YB-1, conjugated to KLH. The corresponding sequence is identical in rat and mouse YB-1 proteins.

Applicazioni

Anti-YB-1 (C-terminal) antibody has been used in

• immunoblotting
• immunoprecipitation
• immunofluorescence
• double-immunostaining

Azioni biochim/fisiol

Y-box-binding protein (YB-1) is shown to bind to the Y-box promoter element to regulate gene expression. It plays an important role in a wide variety of cellular functions such as transcriptional and translation regulation, DNA repair, drug resistance, and stress responses.

Stato fisico

Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)