T7777
TRIS glycine SDS buffe
Sinonimo/i:
TRIS glycine SDS buffer solution
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About This Item
Codice UNSPSC:
41105319
eCl@ss:
32129211
NACRES:
NA.25
Prodotti consigliati
Sterilità
0.2 μm filtered
Livello qualitativo
Stato
liquid
applicazioni
diagnostic assay manufacturing
Temperatura di conservazione
2-8°C
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Descrizione generale
Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.
Applicazioni
Tris-glycine-SDS buffer 10× concentrate has been used as a running buffer in sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE). It is also used to block membranes with 5% non-fat dry milk for western blotting. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.
Ricostituzione
Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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