T7539
Tumor Necrosis Factor-α from mouse
≥95%, recombinant, expressed in E. coli, powder, suitable for cell culture
Sinonimo/i:
mTNF-α, TNF-α
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About This Item
Prodotti consigliati
product name
Tumor Necrosis Factor-α from mouse, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
Origine biologica
mouse
Livello qualitativo
Ricombinante
expressed in E. coli
Saggio
≥95%
Forma fisica
powder
Potenza
0.01-0.5 ng/mL EC50
PM
17 kDa
Confezionamento
pkg of 10 μg
pkg of 50 μg
Condizioni di stoccaggio
avoid repeated freeze/thaw cycles
tecniche
cell culture | mammalian: suitable
Impurezze
≤1 EU/μg protein endotoxin
Colore
white
Solubilità
water: soluble
N° accesso UniProt
Temperatura di conservazione
−20°C
Informazioni sul gene
mouse ... Tnf(21926)
Applicazioni
Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used:
- to activate MS1 endothelium cells
- to induce lipolysis in adipocytes
- to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation
Azioni biochim/fisiol
Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.
Definizione di unità
One unit is the amount required to induce half-maximal cytolysis of L929 cells.
Stato fisico
Lyophilized from 10 mM sodium phosphate, pH 7.5
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Skin Irrit. 2
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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