T2928

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse

clone TH-16, ascites fluid

• Sinonimo/i: Anti-DYT14, Anti-DYT5b, Anti-TYH
• Numero MDL: MFCD00213051
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: ascites fluid
• Tipo di anticorpo: primary antibodies
• Clone: TH-16, monoclonal
• contiene: 15 mM sodium azide
• Reattività contro le specie: rat, sheep, human, monkey, bovine, rabbit, guinea pig
• tecniche: immunohistochemistry: suitable; immunoprecipitation (IP): suitable using native or SDS-denatured TH.; microarray: suitable; western blot: 1:8,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells.
• Isotipo: IgG1
• N° accesso UniProt: P07101
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TH(7054) ; rat ... Th(25085)

Descrizione

Descrizione generale

Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the TH-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Tyrosine hydroxylase (TH) exists in four isoforms in humans. They are are TH-1 (M.W. 55,600), TH-2 (4 additional amino acids; M.W. 56,000), TH-3, (27 additional amino acids; M.W. 58,100), and TH-4 (4 plus 27 additional amino acids; 58,500). Only humans express all four isoforms; all other anthropoids express only the TH-1 and TH-2 isoforms.

Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH₄) and O₂ as cosubstrates, and Fe²⁺ as a cofactor.

Specificità

The antibody recognizes an epitope present in the N-terminal region (between aa 40-152) of both rodent and human tryosine hydroxylase. TH-16 detects both the intact subunits and the 59/57 kDa doublet and an array of decreasing molecular weight TH forms resulting from severe proteolysis.

Immunogeno

rat tyrosine hydroxylase (TH).

Applicazioni

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in

• western blotting
• immunofluorescence labelling
• immunohistochemical staining
• enzyme linked immunosorbent assay (ELISA)
• dot blot
• immunoprecipitation
• immunocytochemistry

Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in immunohistochemistry and double immunofluorescence labeling.

Azioni biochim/fisiol

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable