T1194

Trizma^® Pre-set crystals

BioPerformance Certified, pH 8.5, average M_w 131.4

• Sinonimo/i: Tris crystals
• Codice UNSPSC: 12161700
• NACRES: NA.25

Proprietà

• Grado: BioPerformance Certified
• Livello qualitativo: 200
• Stato: crystalline powder
• PM: average M_w 131.4
• tecniche: cell culture | mammalian: suitable
• Impurezze: DNase, RNase, Protease, NICKase, none detected; endotoxin, tested; total aerobic microbial count, tested; ≤0.05 A₂₉₀; ≤0.5% water (Karl Fischer); ≤5 ppm Heavy metals (as Pb)
• pH: 8.22 (37 °C, 0.1 M)(lit.); 8.50 (25 °C, 0.1 M)(lit.); 8.5; 9.09 (5 °C, 0.1 M)(lit.)
• Intervallo di pH utile: 7.0-9.0
• Solubilità: water: soluble, clear
• applicazioni: diagnostic assay manufacturing

Descrizione

Applicazioni

Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Confezionamento

In convenient foil pouches

Altre note

This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.

Certain pH electrodes do not give accurate results with Tris buffers.

Nota sulla preparazione

Trizma solutions can be autoclaved.

Note legali

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves