SRP2024
PC4, serine mutations human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Sinonimo/i:
MGC102747, P15, PC4, p14
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.26
Prodotti consigliati
Origine biologica
human
Ricombinante
expressed in E. coli
Saggio
≥80% (SDS-PAGE)
Forma fisica
frozen liquid
PM
~14.4 kDa
Confezionamento
pkg of 10 μg
Concentrazione
1000 μg/mL
Colore
clear colorless
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Informazioni sul gene
human ... SUB1(10923)
Azioni biochim/fisiol
Human PC4 is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II in vitro through most tested activators. The function of PC4 is through interactions with transcriptional activators and the basal transcription machinery. It is negatively regulated by casein kinase II phosphorylation both in vitro and in vivo. PC4 strongly binds single stranded DNA and the region essential for the single stranded DNA binding activity was mapped around residue 77. A single amino acid change at position 77 (F to P) abolishes both ds- and ss-DNA binding activity.
Stato fisico
Clear and colorless frozen liquid solution
Nota sulla preparazione
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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H Ge et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(26), 12691-12695 (1994-12-20)
Human positive cofactor 4 (PC4) mediates activator-dependent transcription by RNA polymerase II, apparently through interactions with transcriptional activators and the basal transcription machinery. We report here that PC4 function is modulated by in vivo phosphorylation. Protein-protein interaction studies and in
M Kretzschmar et al.
Cell, 78(3), 525-534 (1994-08-12)
Our investigations of mammalian class II gene transcription resulted in identification, purification, and cloning of the corresponding cDNA of a cellular factor (p15) that mediates the effects of several distinct activators on transcription in vitro. Functional deletion analyses revealed a
H Ge et al.
Cell, 78(3), 513-523 (1994-08-12)
Activator-dependent transcription in mammalian cells requires upstream stimulatory activity (USA)-derived cofactors in addition to those present in TFIID. A novel positive cofactor (PC4) purified from the human USA fraction effected a marked enhancement (up to 85-fold) of GAL4-AH-dependent transcription in
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