SML3867

BI, Broccoli Fluorophore

≥98% (HPLC)

• Sinonimo/i: (5Z)-3-(1H-Benzimidazol-7-ylmethyl)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4H-imidazol-4-one, (Z)-3-((1H-Benzo[d]imadazol-4-yl)methyl)-5-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-3,5-dihydro-4H-imidazol-4-one, BI, DFHBI-BI
• Formula empirica (notazione di Hill): C₁₉H₁₄F₂N₄O₂
• Numero CAS: 2561482-42-4
• Peso molecolare: 368.34
• Codice UNSPSC: 12352200

Proprietà

• Livello qualitativo: 100
• Saggio: ≥98% (HPLC)
• Forma fisica: powder
• Colore: white to beige
• Solubilità: DMSO: 2 mg/mL, clear
• Temperatura di conservazione: room temp

Descrizione

Azioni biochim/fisiol

Cell-permeable DFHBI derivative that selectively binds to Broccoli aptamer; used for imaging of RNA in living cells.



BI is a cell-permeable DFHBI benzimidazolo (DFHBI-BI) derivative that greatly and selectively promotes and stabilizes Broccoli aptamer folding in vitro and cells with enhanced photostability. BI displays little background in vitro and cells (ϕ=0.00048, ϵ=42,500 M−1 cm−1), but very high fluorescence activation after binding to Broccoli (ϕ=0.669, ϵ=33,600 M−1 cm−1, KD = 51 nM). DFHBI-BI shows negligible cellular toxicity and binds Squash with much weaker affinity than Broccoli. Ex/Em: 470/505 nm.