SAE0003

4-Hydroxyphenylpyruvate Dioxygenase from Streptomyces avermitilis

recombinant, expressed in E. coli

• Classificazione EC (Enzyme Commission): 1.13.11.27 (BRENDA, IUBMB)
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Ricombinante: expressed in E. coli
• Livello qualitativo: 200
• Forma fisica: liquid
• Attività specifica: ≥5 U/mg
• PM: ~41.8 kDa
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

4-Hydroxyphenylpyruvate Dioxygenase (4-HPPD) is a key enzyme in the catabolism of tyrosine and phenylalanine. 4-HPPD catalyzes the synthesis of homogenistate (2,5-dihydroxyphenylacetate) from ρ-hydroxyphenylpyruvate and molecular oxygen. 4-HPPD also catalyzes the conversion of α-ketoisocaproate to Β-hydroxyisovalerate. 4-HPPD requires Fe²⁺ as an essential cofactor.

Rat 4-HPPD has a minimum of 80% homology with human 4-HPPD. Rat 4-HPPD is expressed abundantly in hepatocytes. It is also expressed in kidney, particularly in epithelial cells of the proximal tubules and the distant tubules. 4-HPPD has been identified as a potential biomarker of drug-induced liver injury in rat studies.

Definizione di unità

One unit will oxidize 1 μmole of molecular oxygen per minute at pH 7.0, 25 °C, in the presence of 4-hydroxyphenylpyruvate.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable