SAB4600388

Anti-Mouse IgG (H+L), F(ab′)2 fragment, CF^™488A antibody produced in goat

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

• Codice UNSPSC: 12352203
• NACRES: NA.46

Proprietà

• Origine biologica: goat
• Livello qualitativo: 100
• Coniugato: CF^™ 488A conjugate
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: secondary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• Reattività contro le specie: mouse
• Concentrazione: ~2 mg/mL
• tecniche: flow cytometry: 1-10 μg/mL; immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: 1-10 μg/mL; western blot: suitable
• Fluorescenza: λ_ex 490 nm; λ_em 515 nm
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Digestion of IgG by papain results in generation of fragment antigen binding (Fab), comprising of one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallizable (fc), comprising of the H chain constant region. IgG1 is the most abundant antibody and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection. IgG antibody has enormous therapeutic potential and the Fc region is used for the development of therapeutic antibodies.

This product is prepared by labeling high quality F(ab′)2 fragment of goat anti-mouse IgG (H+L) with the green fluorescent dye CF488A. CF488A is a superior alternative to the traditional green fluorescent dye FITC (or fluorescein) owing to its exceptional brightness and photostability. CF488A is comparably bright and photostable to Alexa Fluor 488. However, antibody conjugates prepared from CF488A are more specific than those prepared from Alexa Fluor 488 because CF488A, being minimally charged, does not significantly change the isoelectric points of antibodies as Alexa Fluor 488 does.

Specificità

Binds with whole molecule mouse IgG and the light chains of other mouse immunoglobulins.

Immunogeno

mouse IgG(H+L)

Applicazioni

Anti-Mouse IgG (H+L), F(ab?)2 fragment, CF^™488A antibody produced in goat has been used in the detection of CF488 in human cell lines using confocal fluorescence microscopy and in vinculin cytoskeleton using confocal immunofluorescence imaging.

Caratteristiche e vantaggi

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Stato fisico

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

Nota sulla preparazione

Protect from light. The antibody solution should be gently mixed before use.

Note legali

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

CF is a trademark of Biotium, Inc.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2