SAB4300123

Anti-phospho-JAK1 (pTyr¹⁰²²) antibody produced in rabbit

affinity isolated antibody

• Sinonimo/i: Anti-JAK1A antibody produced in rabbit, Anti-JAK1B antibody produced in rabbit, Anti-JTK3 antibody produced in rabbit, Anti-Janus kinase 1 (a protein tyrosine kinase) antibody produced in rabbit
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: ~130 kDa
• Reattività contro le specie: rat, mouse, human
• Concentrazione: 1 mg/mL
• tecniche: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100; western blot: 1:500-1:1000
• Isotipo: IgG
• Sequenza immunogenica: (K-E-YP-Y-T)
• N° accesso NCBI: NP_002218.2
• N° accesso UniProt: P23458
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: phosphorylation (pTyr1022)
• Informazioni sul gene: human ... JAK1(3716)

Descrizione

Descrizione generale

Janus kinase 1 (JAK1) is encoded by the gene mapped to human chromosome 1p31.3. The encoded protein is one of the four members of the mammalian JAK families. Members of JAK family contain an N-terminal FERM (four.1, ezrin, radixin, moesin) domain, through which they interact with cytokine receptors. The N-terminal end also contains an SH2-like domain. In addition, it also contains a kinase domain and a pseudo-kinase domain, which is catalytically inactive. JAK1 is ubiquitously expressed. When expressed without their receptors, JAKs reside in the cytosol. However, under normal cell conditions, they localize to plasma membrane and endosomes.

Immunogeno

Peptide sequence around phosphorylation site of tyrosine 1022 (K-E-Y(p)-Y-T), according to the protein JAK1.

Applicazioni

Anti-phospho-JAK1 (pTyr1022) antibody produced in rabbit has been used in Western blot analysis.

Azioni biochim/fisiol

Janus kinases (JAKs) are activated when cytokines bind with their receptors, upon which they phosphorylate the receptors and produce docking sites for signaling molecules. One of the main signaling molecules includes signal transducer and activator of transcription (Stat) family. JAK1 is needed by multiple cytokines, including the family using the shared receptor subunit called common γ chain (γc), such as interleukin (IL)-2, IL-4, IL-7, IL-9, IL-15 and IL-21. JAK1 KO (knockout) mice have early postnatal lethal or perinatal lethal phenotype due to neurological defects. In vitro studies show that JAK1S703I mutation functions as an activator of JAK-STAT pathways even in the absence of cytokines, and drives cellular proliferation. JAK1S703I mutant patient-derived xenograft (PDX) HCC (hepatocellular carcinoma) cells also show sensitivity to JAK1/2 inhibitor, ruxolitinib.

Caratteristiche e vantaggi

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Descrizione del bersaglio

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

Stato fisico

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable