S9822

Anti-Sonic Hedgehog peptide (C-terminal) antibody produced in goat

affinity isolated antibody, lyophilized powder

• Sinonimo/i: Anti-Dsh, Anti-Hhg1, Anti-Hx, Anti-Shh
• Codice UNSPSC: 51111800
• NACRES: NA.41

Proprietà

• Origine biologica: goat
• Livello qualitativo: 100
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: lyophilized powder
• Reattività contro le specie: mouse
• tecniche: immunohistochemistry: 15 μg/mL; western blot: 0.1-0.2 μg/mL
• N° accesso UniProt: Q62226
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Shh(20423)

Descrizione

Descrizione generale

The Hedgehog (Hh) family of secreted proteins consists of sonic hedgehog (Shh), desert hedgehog (Dhh) and Indian hedgehog (Ihh). The Hh signaling regulates cell migration and tissue construction during embryogenesis and reconstruction in adults. Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, γ catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system. Hedgehog signaling has been implicated in childhood tumors of pancreas, liver, thyroid, gastrointestinal tract, neuroblastomas and medulloblastomas
Anti-Sonic Hedgehog Peptide (C-terminal) recognizes mouse Sonic Hedgehog C-terminal peptide. This antibody shows less than 5% cross-reactivity with rmDhh C-terminal peptide and less than 1% cross-reactivity with a 6X histidine-tagged rmShh N-terminal peptide (amino acids 25-198).

Immunogeno

purified, E. coli-derived recombinant mouse 6× histidine-tagged sonic hedgehog C-terminal peptide.

Applicazioni

Anti-Sonic Hedgehog Peptide (C-terminal) can be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For immunohistochemistry, a working concentration of 15 μg/ml is recommended.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)