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S500HR

Sigma-Aldrich

Sephacryl® Size Exclusion Resin

500-HR, MW range 40-20,000 kDa (dextrans)

Sinonimo/i:

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

Numero CAS:
65546-95-4
Numero MDL:
MFCD00165824
Codice UNSPSC:
23151817
NACRES:
NA.56

Nome del prodotto

Sephacryl®, 500-HR, MW range 40-20,000 kDa (dextrans)

Livello qualitativo

100

Descrizione

stability: stable to 0.2M NaOH

Stato

suspension (20% aqueous ethanol)

Caratteristiche

autoclavable

Matrice

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

Dimensione particelle

25-75 μm (wet)

Dimensione pori

40-20,000 kDa MW range (dextrans)

Compatibilità

mode of use size exclusion chromatography

Temperatura di conservazione

2-8°C

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Descrizione generale

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S500HR-750ML′s updated product number is GE17-0613-01

Applicazioni

Sephacryl® has been used in size exclusion chromatography purification of the Acacia tortuosa gum, dextran-RBA12 from Weissella cibaria and dextran from Weissella cibaria JAG8.
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Note legali

Sephacryl is a registered trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Classi di pericolo

Flam. Liq. 3

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

100.4 - 109.4 °F - closed cup

Punto d’infiammabilità (°C)

38 - 43 °C - closed cup

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
MKBS9866V
MKBM8415V
SLBB6826V
109K1525
068K1391

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Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

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M A Karymov et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(14), 2631-2641 (2001-12-01)
Dynamic alterations in chromatin structure mediated by postsynthetic histone modifications and DNA methylation constitute a major regulatory mechanism in DNA functioning. DNA methylation has been implicated in transcriptional silencing, in part by inducing chromatin condensation. To understand the methylation-dependent chromatin
Theresa L Gioannini et al.
The Journal of biological chemistry, 277(49), 47818-47825 (2002-10-10)
Experiments utilizing endotoxin aggregates, lipooligosaccharides (LOS) isolated from metabolically labeled Neisseria meningitidis serotype group B, demonstrate that albumin is an essential component of lipopolysaccharide binding protein- (LBP) and sCD14-dependent 1) disaggregation of LOS and 2) LOS activation of human umbilical
Structural and biocompatibility properties of dextran from Weissella cibaria JAG8 as food additive
Tingirikari JM, et al.
International Journal of Food Sciences and Nutrition, 65(6), 686-691 (2014)
Xiaolei Wang et al.
Molecular microbiology, 83(1), 195-207 (2011-11-25)
Stewartan and amylovoran exopolysaccharide (EPS) produced by the plant pathogenic bacteria Pantoea stewartii and Erwinia amylovora are virulence factors in the cause of Stewart's vascular wilt and fire blight. The biosynthesis of amylovoran and stewartan is encoded by a set
Takashi Ideue et al.
Genes & development, 21(16), 1993-1998 (2007-08-07)
Pre-mRNA splicing specifically deposits the exon junction complex (EJC) onto spliced mRNA, which is important for downstream events. Here, we show that EJC components are primarily recruited to the spliceosome by association with the intron via the intron-binding protein, IBP160.
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