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PROTRA

Millipore

ProteoPrep® Reduction and Alkylation Kit

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Durata

≥1 yr (when stored at 2-8°C)

Livello qualitativo

200

Temperatura di conservazione

2-8°C

Applicazioni

The ProteoPrep Reduction & Alkylation Kit contains the necessary reagents to conveniently reduce and alkylate disulfide binds in preparation for 2D gel electrophoresis. These reagents are compatible with chaotropic extraction reagents and are conveneintly packaged to save time and increase efficiency. The kit contains five vials of both the reducing agent, tributylphosphine (TBP), and the alkylating agent, iodoacetamide.

Caratteristiche e vantaggi

  • Tributylphosphine is supplied safely as a 200 mM solution in N-methyl-2-pyrrolidine
  • Reduction and alkylation of protein samples increase 2D spot resolution
  • Conveniently packaged components save time and increase efficiency
  • Compatibility with chaotropic extraction reagents simplifies sample preparation

Note legali

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Avvertenze

Danger

Classi di pericolo

Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Iodoacetamide BioUltra

Sigma-Aldrich

I1149

Iodoacetamide

Tripsina TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

Sigma-Aldrich

T1426

Tripsina

DL-Ditiotreitolo BioXtra, ≥99.0% (titration)

Sigma-Aldrich

D5545

DL-Ditiotreitolo

Puntali per pipette ZipTip® C18 resin, bed volume 0.6 μL, tip volume 10 μL

Millipore

ZTC18S

Puntali per pipette ZipTip®

Biotina ≥99% (HPLC), lyophilized powder

Sigma-Aldrich

B4501

Biotina

Shikha Shikha et al.
Microorganisms, 10(5) (2022-05-29)
Mitochondrial ribosomes are fundamental to mitochondrial function, and thus survival, of nearly all eukaryotes. Despite their common ancestry, mitoribosomes have evolved divergent features in different eukaryotic lineages. In apicomplexans, the mitochondrial rRNA is extremely fragmented raising questions about its evolution

Articoli

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

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