P5542
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus
lyophilized powder, ≥1,000 units/mg protein (Lowry)
Sinonimo/i:
PC-PLC
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About This Item
Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.32
Prodotti consigliati
Stato
lyophilized powder
Livello qualitativo
Attività specifica
≥1,000 units/mg protein (Lowry)
Attività estranea
Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein
Temperatura di conservazione
−20°C
Informazioni sul gene
Bacillus cereus E33L ... BCZK3513(3026815)
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Descrizione generale
Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.
Applicazioni
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.
Azioni biochim/fisiol
Used for the release of GPI anchored proteins from the membrane.
Definizione di unità
One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).
Stato fisico
Contains phosphate buffer salts, EDTA and stabilizer
Risultati analitici
Acetylcholinesterase is measured according to Ellman, et al.
Inibitore
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Articoli
GPI Anchored Glycoproteins
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