N8773

Anti-Nerve Growth Factor-β antibody produced in goat

affinity isolated antibody, lyophilized powder

• Sinonimo/i: Anti-NGF-β
• Numero MDL: MFCD00282385
• Codice UNSPSC: 51111800
• NACRES: NA.41

Proprietà

• Origine biologica: goat
• Livello qualitativo: 100
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: lyophilized powder
• Reattività contro le specie: rat
• tecniche: capture ELISA: 0.2-0.8 μg/mL; immunohistochemistry: 5-15 μg/mL; neutralization: 0.02-0.1 μg/mL; western blot: 0.1-0.2 μg/mL
• N° accesso UniProt: P25427
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: rat ... Ngf(310738)

Descrizione

Descrizione generale

Nerve Growth factor (NGF) is a growth factor that is essential for the development and maintenance of the nervous system. NGF is composed of three non-covalently linked α, β and γ subunits. The β subunit is responsible for the biological functions of NGF. Nerve growth factor (NGF) binds to either to NGF receptor (NGFR) or the Trk receptor and can elicit biological responses through each of the receptors independently. NGF is important in the survival and differentiation of neurons and neural crest-derived cells during embryonic and early postnatal development and maintenance of peripheral neurons. NGF is also implicated in inflammation and allergic reactions . Anti-Nerve Growth Factor-β antibody recognizes rat NGF-β. The antibody shows less than 1% cross reactivity with recombinant human β-NGF.

in the presence of 10.0μg/ml of recombinat rat β-NGF using the human erythroleukemic TF-1 cell line.

Immunogeno

recombinant rat β-nerve growth factor expressed in Sf 21 cells.

Applicazioni

Anti-NGF-β antibody may be used for neutralization assays; the ND50 is ~0.02-0.1 μg/ml. For immunoblotting the recommended antibody concentration is 0.1-0.2 μg/ml. The antibody is suitable for immunohistochemistry at a working concentration of 5-15 μg/ml. For ELISA, antibody concentration of 0.2-0.8 μg/ml may be used. Antibody dilution of 1:500 was used for immunoblotting of extracts of rat nerve cells.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable