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Key Documents

N3910

Sigma-Aldrich

Neutralization Solution B

Sinonimo/i:

DNA neutralization solution, neutralization buffer DNA extraction

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About This Item

Codice UNSPSC:
12161703
NACRES:
NA.25

Grado

for molecular biology

Forma fisica

liquid

tecniche

DNA amplification: suitable
DNA extraction: suitable

Temperatura di conservazione

−20°C

Descrizione generale

This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.

Applicazioni

Neutralization Solution B has been used in the modified Sigma Extract-N-Amp ™ extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp™ PCR Reaction Mix.

Note legali

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Pittogrammi

Exclamation markEnvironment
GHS07,GHS09

Avvertenze

Warning

Indicazioni di pericolo

H315,H317,H319,H411

Classi di pericolo

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol

The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

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