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N2513

Sigma-Aldrich

Nerve Growth Factor β rat

≥97% (SDS-PAGE), recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture

Sinonimo/i:

NGF-β from rat, Nerve Growth Factor-β from rat

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About This Item

Numero CAS:
Numero MDL:
Codice UNSPSC:
12352202
NACRES:
NA.77

Nome del prodotto

NGF-β from rat, recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture

Origine biologica

rat

Livello qualitativo

Ricombinante

expressed in Sf21 cells

Saggio

≥97% (SDS-PAGE)

Stato

lyophilized powder

Potenza

0.15-3.00 ng/mL EC50

Confezionamento

pkg of 0.1 mg

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Solubilità

water: soluble 0.1 g/L at 20 °C

N° accesso UniProt

Temperatura di conservazione

−20°C

Informazioni sul gene

rat ... NGF(4803)

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Descrizione generale

NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells. It is a neurotrophin and belongs to the NGF family.

Applicazioni

NGF-β from rat is used for the following applications:
  • In the preparation of DMEM medium (for the cultivation and differentiation of PC12 cells)
  • Western blotting analysis
  • For the preparation of differentiation medium (it is prepared from DMEM, in which NGF-β is one of the components)
  • In cell culture and treatment (for neuronal differentiation)
  • In the preparation of stock solution

Azioni biochim/fisiol

NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers. It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same. It might be involved in changes in placental function, as NGF signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine

Risultati analitici

The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1.

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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