MSKT0004

IA-MS Immunoaffinity Mass Spectrometry Kit - Protein A/G

96 Well Strip Plate with SOLu-Trypsin

• Sinonimo/i: Target protein enrichment and quantification from complex background by mass spectrometry.
• Codice UNSPSC: 41115859
• NACRES: NA.32

Proprietà

• Forma fisica: solid
• Compatibilità: suitable for mass spectrometry
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

The Protein A/G IA-MS Immunoaffinity Mass Spectrometry Kit is a 96-well platform enabling robust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS. Users provide their own capture antibody and internal standard (available separately, internal standards may be SIL peptides or preferably full length SIL protein) and the kit contains all other components needed for in-well enrichment and rapid trypsin digestion of samples. The plate format consists of 8-well strips allowing for partial runs, and avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. The entire workflow can be performed in under 5 hours, resulting in digests ready for injection with no SPE or other cleanup required.

Applicazioni

Target protein enrichment and quantification from complex background by mass spectrometry.

Caratteristiche e vantaggi

• Robust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS
• Entire workflow including digest completed in under 5 hours, such that samples are injection ready at the end of the work day
• 96-well plate format has potential for automation in a liquid-handling system
• Bead-free system circumvents messy clean-up and eliminates risk of column clogging
• Plate strips allow user to run as many or as few samples as desired

Confezionamento

Box with foam insert containing kit components.

Componenti

Protein A/G Coated Plates SAE0113-1EA
SOLu-Trypsin EMS0004-100UG
MS Denaturation Solution EMS00010-10ML
Rapid Trypsin Digestion Buffer EMS0009-30ML
Tris Buffered Saline with TWEEN^® 20 (TBST)powder, pH 8.0 T9039
Tris Buffered Saline (TBS) powder, pH 8.0 T6664
EZ-Pierce^™ plate seal Z721581-1EA

See datasheet for more information.

Stoccaggio e stabilità

2 years when stored at 2-8 °C.

Related Presentation

• Development and Comparison of Two High Throughput LC-MS Methods for the Accurate Quantitation of IGF1 in Human Serum (Poster, PDF)

Note legali

EZ-Pierce is a trademark of Excel Scientific, Inc.

TWEEN is a registered trademark of Croda International PLC

Solo come componenti del kit

• MS Denaturation Solution
• Rapid Trypsin Digestion Buffer

I componenti del kit sono disponibili anche separatamente

• Protein A/G Coated Plates
• EMS0004 SOLu-Trypsin, recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid
• T9039 Tris Buffered Saline, with Tween™ 20, pH 8.0, powder
• T6664 Tris Buffered Saline, pH 8.0, powder
• Z721581 EZ-Pierce^™ films, Excel Scientific EZP-100, non-sterile

Informazioni sulla sicurezza

• Pittogrammi: GHS02,GHS06,GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H226,H301 + H311 + H331,H370
• Consigli di prudenza: P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311
• Classi di pericolo: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 3 - STOT SE 1
• Organi bersaglio: Eyes,Central nervous system
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Punto d’infiammabilità (°F): 75.2 °F
• Punto d’infiammabilità (°C): 24 °C