M5057
MES
BioReagent, ≥99% (titration), crystalline
Sinonimo/i:
Acido 2-(N-morfolino)etansolfonico, Acido 4-morfolin-etansolfonico
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About This Item
Formula empirica (notazione di Hill):
C6H12NNaO4S
Numero CAS:
Peso molecolare:
217.22
Beilstein:
3765682
Numero CE:
Numero MDL:
Codice UNSPSC:
12161700
eCl@ss:
32129211
ID PubChem:
NACRES:
NA.25
Prodotti consigliati
Nome Commerciale
BioReagent
Livello qualitativo
Saggio
≥99% (titration)
Stato
crystalline
pH
8.9-10.1 (1% in water)
Intervallo di pH utile
5.5-6.7
pKa
6.1
Solubilità
water: 0.5 g/mL, clear, colorless
applicazioni
diagnostic assay manufacturing
Temperatura di conservazione
room temp
Stringa SMILE
[Na+].[O-]S(=O)(=O)CCN1CCOCC1
InChI
1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1
IRHWMYKYLWNHTL-UHFFFAOYSA-M
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Categorie correlate
Descrizione generale
MES is one of the so-called "Good" buffers developed for biological applications. This product is tested for trace ions. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±-1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
Applicazioni
MES sodium salt has been used:
- To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNA
- To equilibrate the Balch homogenizer chamber and to prevent sample hydrolysis before sample homogenization
Nota sulla preparazione
MES Sodium is soluble in water, giving a clear colourless solution at 25g in 50mL water (approximately 2.3M). The pH of a solution should be between 9 and 10 depending on concentration. Solutions should be stable at 2-8°C for months. Sterilization should be by filteration through 0.2μm filters. Autoclaving is not recommended. If buffers must be nuclease-free, it is best to treat the water first, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.
Altre note
Easily compare specifications for MES sodium salt products with the MES sodium salt specification table.
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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