M042030

Biotin Phosphoramidite

configured for ABI

• Sinonimo/i: Biotin amidite
• Formula empirica (notazione di Hill): C₄₆H₆₄N₅O₈PS
• Numero CAS: 1217500-22-5
• Peso molecolare: 878.07
• Numero MDL: MFCD00676535
• Codice UNSPSC: 41116105
• ID PubChem: 329817780
• NACRES: NA.21

Proprietà

• Nome Commerciale: Proligo Reagents
• Livello qualitativo: 200
• Saggio: ≥96% (³¹P-NMR)
• Compatibilità: conforms to structure for H-NMR; configured for ABI
• Temperatura di conservazione: −20°C
• Stringa SMILE: COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1; WSDQYLGSIUJJRU-ZMKGMYJVSA-N

Descrizione

Descrizione generale

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis. Biotin-labelled oligonucleotides have become extremely popular in DNAcapture and detection applications, due to their very high-sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotinbased detection methods and are widely used by researchers. Biotin labelling is compatible with PCR and most hybridization techniques.

Key Features of Biotin Phosphoramidite

• Behaves like any standard phosphoramidite on the DNA synthesizer
• Readily soluble in acetonitrile
• Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification
• A long, water-compatible linker arm ensures maximum sensitivity
• Provides a coupling efficiency of at least 95%The product is configured for ABI^™ Synthesizers

Note legali

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable