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Key Documents

K4377

Sigma-Aldrich

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse

clone KP-53, ascites fluid

Sinonimo/i:

Monoclonal Anti-Human Kappa Light Chain (Bound and Free)

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

secondary antibodies

Clone

KP-53, monoclonal

contiene

15 mM sodium azide

tecniche

indirect ELISA: 1:10,000

Isotipo

IgG1

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Descrizione generale

Monoclonal Anti-Human Kappa Light Chain (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins G are made up of two identical light chains and two identical heavy chains, that are covalently connected by interchain disulphide bonds. Genes of kappa and lambda constant region are mapped on to human chromosome 2.

Specificità

The antibody is specific for human kappa (κ) light chains. This antibody is reactive with intact IgG (κ), IgM (κ), free kappa light chains, and Bence-Jones kappa light chains. It is non-reactive with lambda light chains.

Applicazioni

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse has been used for:
  • immunodetection
  • in western blot analysis
  • in intracellular kappa light chain staining for flow cytometric analysis

Stato fisico

Monoclonal Anti-Human Kappa Light Chain (Bound and Free) antibody produced in mouse is provided as ascites fluid with 0.1% sodium azide (see MSDS) as a preservative.

Stoccaggio e stabilità

For continuous use, store at 2-8 °C. For extendedstorage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy
Mathias S, et al.
Journal of Biotechnology, 271, 47-55 (2018)
Upregulated expression of kappa light chain by Epstein-Barr virus encoded latent membrane protein 1 in nasopharyngeal carcinoma cells via NF-kappaB and AP-1 pathways
Liu H, et al.
Cellular Signalling, 19(2), 419-427 (2007)
Chloe L Stoyle et al.
The Biochemical journal, 474(18), 3179-3188 (2017-08-09)
Rodent monoclonal antibodies with specificity towards important biological targets are developed for therapeutic use by a process of humanisation. This process involves the creation of molecules, which retain the specificity of the rodent antibody but contain predominantly human coding sequence.
Heterogeneity of aberrant immunoglobulin expression in cancer cells
Hu D, et al.
Cellular & Molecular Immunology, 8(6), 479-479 (2011)
Expression and secretion of immunoglobulin alpha heavy chain with diverse VDJ recombinations by human epithelial cancer cells
Zheng H, et al.
Molecular Immunology, 44(9), 2221-2227 (2007)

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