H9773

Igromicina B

lyophilized powder, suitable for plant cell culture, BioReagent

• Sinonimo/i: Hygromycin
• Formula empirica (notazione di Hill): C₂₀H₃₇N₃O₁₃
• Numero CAS: 31282-04-9
• Peso molecolare: 527.52
• Numero CE: 250-545-5
• Numero MDL: MFCD06795479
• Codice UNSPSC: 12352207
• ID PubChem: 329815270
• NACRES: NA.72

Proprietà

• product name: Igromicina B, suitable for plant cell culture, BioReagent, ≥60% (HPLC), lyophilized powder
• Origine biologica: Streptomyces hygroscopicus
• Livello qualitativo: 200
• Nome Commerciale: BioReagent
• Forma fisica: lyophilized powder
• Purificato mediante: ion-exchange chromatography
• Concentrazione: ≥60% (HPLC)
• tecniche: cell culture | plant: suitable
• Colore: faintly brown to brown; white to beige
• Solubilità: H₂O: soluble 50 mg/mL; ethanol: soluble; methanol: soluble
• Spettro attività antibiotica: fungi
• applicazioni: agriculture
• Modalità d’azione: protein synthesis | interferes
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1; GRRNUXAQVGOGFE-XKIAHZFYSA-N

Descrizione

Descrizione generale

Chemical structure: aminoglycoside

Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

Applicazioni

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Hygromycin B from Streptomyces hygroscopicus is used for gene cloning and ectopic expression. It is also used in MS medium for the in vitro screening of transgenic plants.

Azioni biochim/fisiol

Hygromycin B is used in mutagenesis or for selection of gene expression and complementation constructs in C. jejuni. It is also adopted as a marker in other approaches for manipulating, introducing or deleting DNA in C. elegans. It can be inactivated by Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) through phosphorylation. The amino function of this antibiotic may be involved in a general acid-base catalysis performed by the ribozyme, acting as proton acceptors/donors.

Modalità d′azione: Il prodotto inibisce la sintesi proteica inducendo un′errata lettura del templato di m-RNA nel procariota che può inibire la traduzione.

Spettro antimicrobico: L′igromicina B agisce contro batteri, funghi e cellule eucariote superiori.

Avvertenza

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is table for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8°C.

Nota sulla preparazione

This product is purified by ion exchange chromatography. Hygromycin B is soluble in water at concentrations >50 mg/mL, and also soluble in methanol or ethanol. Solutions should be sterilized by filtration, not by autoclaving. Additionally, Hygromycin B solutions have been reported to lose activity on freezing, and since they are stable refrigerated, freezing should be avoided.

Informazioni sulla sicurezza

• Pittogrammi: GHS06
• Avvertenze: Danger
• Indicazioni di pericolo: H300 + H310 + H330
• Consigli di prudenza: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Classi di pericolo: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Codice della classe di stoccaggio: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable