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H8651

Sigma-Aldrich

HEPES

BioXtra, ≥99.0% (titration)

Sinonimo/i:

Acido 4-(2-idrossietil)piperazina-1-etansolfonico, N-(2-idrossietil)piperazina-N′-(acido 2-etansolfonico)

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About This Item

Formula empirica (notazione di Hill):
C8H17N2NaO4S
Numero CAS:
75277-39-3
Peso molecolare:
260.29
Numero CE:
278-169-7
Numero MDL:
MFCD00036463
Codice UNSPSC:
12161700
ID PubChem:
24895527
NACRES:
NA.25

Nome Commerciale

BioXtra

Saggio

≥99.0% (titration)

Forma fisica

powder

Impurezze

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

pH

10.0-12.0 (1 M in H2O)

Intervallo di pH utile

6.8-8.2

pKa (25 °C)

7.5

Solubilità

H2O: 1 M, clear, colorless

Anioni in tracce

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

Cationi in tracce

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

Assorbimento

≤0.2 at 260 in H2O at 1 M
≤0.2 at 280 in H2O at 1 M

applicazioni

diagnostic assay manufacturing

Stringa SMILE

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
RDZTWEVXRGYCFV-UHFFFAOYSA-M

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Descrizione generale

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Applicazioni

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Altre note

Easily compare specifications for HEPES sodium salt products with the HEPES sodium salt specification table.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Sigma-Aldrich

H4034

HEPES

HEPES hemisodium salt ≥99% (titration)

Sigma-Aldrich

H7637

HEPES hemisodium salt

HEPES hemisodium salt dry powder

Sigma-Aldrich

H9897

HEPES hemisodium salt

HEPES anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD002

HEPES

HEPES Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1428

HEPES

Soluzione tampone HEPES 1 M in H2O

Sigma-Aldrich

83264

Soluzione tampone HEPES

C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor
I J Chopra et al.
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
Hydrogen ion buffers for biological research.
Good NE, et al.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
Rakesh K Pathak et al.
Analytical chemistry, 84(11), 5117-5123 (2012-05-04)
An in situ prepared Zn(2+) complex of triazole linked imino-thiophenyl conjugate of calix[4]arene, [ZnL], was demonstrated to be highly fluorescent in HEPES buffer solution. [ZnL] has been used as a chemo-sensing ensemble for the recognition of phosphates in general and
Jemimah Walker et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 100(4), 1134-1141 (2012-02-15)
Magnesium (Mg) and its alloys have been proposed as degradable replacements to commonly used orthopedic biomaterials such as titanium alloys and stainless steel. However, the corrosion of Mg in a physiological environment remains a difficult characteristic to accurately assess with
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