H6663
Anti-Histone Deacetylase 7 (HDAC7) antibody, Mouse monoclonal
clone HDAC7-97, purified from hybridoma cell culture
Sinonimo/i:
Anti-HDAC7
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About This Item
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
purified from hybridoma cell culture
Tipo di anticorpo
primary antibodies
Clone
HDAC7-97, monoclonal
Stato
buffered aqueous solution
PM
antigen ~105 kDa
Reattività contro le specie
human, mouse
tecniche
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using recombinant protein from embryonal kidney 293T cells over-expressing human HDAC7
Isotipo
IgG1
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... HDAC7(51564)
mouse ... Hdac7(56233)
Descrizione generale
Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2 and HDAC3 belong to Class I HDACs and HDAC4, HDAC6, and HDAC7 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. HDAC7 is predominantly found in the cell nucleus and slightly in cytoplasm. HDAC7 shuttles between these two regions and this is controlled by 14-3-3 protein and calmodulin-dependent kinase I (CaMKI)
Monoclonal Anti-Histone Deacetylase 7 (HDAC7) (mouse IgG1 isotype) is derived from the hybridoma HDAC7-97 produced by the fusion of mouse myeloma cells (NS1cells) and splenocytes from BALB/c mice. Histone deacetylases (HDACs) are part of transcriptional corepressor complexes. HDAC7 is a member of Mammalian HDAC family. It is localized mainly to the cell nucleus, it is also found in the cytoplasm.
Applicazioni
Anti-Histone Deacetylase 7 (HDAC7) antibody, Mouse monoclonal has been used in:
- chromatin immunoprecipitation
- immunoblotting
- immunocytochemistry
- immunoprecipitation
- ELISA
Monoclonal Anti-Histone Deacetylase 7 (HDAC7) antibody produced in mouse is suitable for:
- immunocytochemistry
- immunoprecipitation
- indirect ELISA
- microarray
- western blot at a concentration of 0.25-0.5μg/mL using cell extracts of human embryonal kidney 293T cells expressing recombinant human HDAC7
Azioni biochim/fisiol
HDAC7 belongs to class II of HDAC family and combines with MEF2 group of transcription factors, which is facilitated by theN-terminal domain of HDAC7. This binding blocks the transcriptional activity and prevents myogenesis. They are involved in development and differentiation processes in various tissues such as skeletal, cardiac, and smooth muscle, the bone, the immune system, the vascular system, and the brain. These signal-dependent co-repressors phosporylate at the regulatory N-terminal domain resulting in nuclear export.
Shuttling of HDAC7 between the cell nucleus and the cytoplasm is controlled by a mechanism involving calmodulin dependent kinase I (CaMKI) and 14-3-3 proteins. The HDAC7 enzymatic activity depends on its interaction with the class I HDAC3, and the corepressors SMRT and N-CoR. HDAC7 also interacts with the transcriptional repressor BCL.
Stato fisico
Solution in 0.01 M phosphte buffered saline, pH 7.4, containing 15 mM sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Human HDAC7 Histone Deacetylase Activity Is Associated with HDAC3in Vivo
Fischle W, et al.
Test, 276(38), 35826-35835 (2001)
Stress-induced epigenetic transcriptional memory of acetylcholinesterase by HDAC4
Sailaja BS, et al.
Proceedings of the National Academy of Sciences of the USA, 109(52), E3687-E3695 (2012)
U Dressel et al.
The Journal of biological chemistry, 276(20), 17007-17013 (2001-03-30)
The overlapping expression profile of MEF2 and the class-II histone deacetylase, HDAC7, led us to investigate the functional interaction and relationship between these regulatory proteins. HDAC7 expression inhibits the activity of MEF2 (-A, -C, and -D), and in contrast MyoD
H Y Kao et al.
The Journal of biological chemistry, 276(50), 47496-47507 (2001-10-05)
Here we show that HDAC7, a member of the class II histone deacetylases, specifically targets several members of myocyte enhancer factors, MEF2A, -2C, and -2D, and inhibits their transcriptional activity. Furthermore, we demonstrate that DNA-bound MEF2C is capable of recruiting
W Fischle et al.
The Journal of biological chemistry, 276(38), 35826-35835 (2001-07-24)
Histone deacetylases (HDACs) are part of transcriptional corepressor complexes and play key roles in regulating chromatin structure. Three different classes of human HDACs have been defined based on their homology to HDACs found in Saccharomyces cerevisiae: RPD3 (class I), HDA1
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
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