GERPN3682
CDP-Star®
Cytiva RPN3682
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About This Item
Prodotti consigliati
Stato
liquid
Confezionamento
pkg of 1 kit
Produttore/marchio commerciale
Cytiva RPN3682
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star detection reagent allows for chemiluminescent detection of Alkaline Phosphatase labelled nucleic acids.
Sufficient reagent for 2500cm² of membrane. Can be used in conjunction with AlkPhos Direct Labeling Module (RPN3680), Amersham AlkPhos Direct Hybridization Buffer (RPN3688) and Hybond/ Nytran membranes.
Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500 cm2 or 5000 cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star or ECF™ detection reagents.
Sufficient reagent for 2500cm² of membrane. Can be used in conjunction with AlkPhos Direct Labeling Module (RPN3680), Amersham AlkPhos Direct Hybridization Buffer (RPN3688) and Hybond/ Nytran membranes.
Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500 cm2 or 5000 cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star or ECF™ detection reagents.
Applicazioni
CDP-Star® has been used to label and quantify genomic DNA by Southern blotting.
Caratteristiche e vantaggi
- Probes are labelled during a 30 minute reaction, resulting in probes that can be used without purification.
- Hybridization stringency can be controlled by temperature as well as salt concentration. Detection is rapid, taking only 30 minutes from completion of hybridization to application of detection reagent.
- Detection is rapid, taking only 30 minutes from completion of hybridization to application of detection reagent.
Stoccaggio e stabilità
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Risultati analitici
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Note legali
CDP-Star is a registered trademark of Tropix, Inc.
ECF is a trademark of Cytiva
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
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Articoli
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
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