GE17-5282-02
GSTrap™ High Performance
Cytiva 17-5282-02, pack of 5 × 5 mL
About This Item
Prodotti consigliati
ligand
glutathione
Confezionamento
pack of 5 × 5 mL
Produttore/marchio commerciale
Cytiva 17-5282-02
Parametri
<20 mL/min flow rate
42 psi (H2O at room temperature.)
Dimensioni del letto
16 mm × 25 mm
Volume del letto
5 mL
Diam. int. colonna
16 mm
Matrice
highly cross-linked 6% agarose
Dimens. media delle particelle
34 μm
cleaning
3-12
working range
3-12
Capacità
>7 mg/mL binding capacity (medium)
Categorie correlate
Descrizione generale
GSTrap™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose™ High Performance. GST-tagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.
GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
Caratteristiche e vantaggi
- High resolution purification of GST-tagged proteins.
- Mild elution conditions preserving protein antigenicity and function.
- Fast, one-step purification.
- Easy to use with syringe, pump or a chromatography system.
Stoccaggio e stabilità
Risultati analitici
Altre note
Note legali
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
Certificati d'analisi (COA)
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Articoli
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.
Protocolli
This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.
Characteristics of Glutathione Sepharose® Products
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