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Key Documents

GE29-0510-21

HisTrap High Performance

Cytiva 29-0510-21, pack of 1 mL

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

Confezionamento

pack of 1 mL

Produttore/marchio commerciale

Cytiva 29-0510-21

Disponibilità

not available in North America

Parametri

flow rate
4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)
42 psi (H2O at room temperature.)

Dimensioni del letto

7 mm × 25 mm

Volume del letto

1 mL

Diam. int. colonna

7 mm

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium)

Capacità

≥40 mg binding capacity (histidine-tagged protein/ml medium)(H2O at room temperature.)
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

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Descrizione generale

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Applicazioni

HisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

Caratteristiche e vantaggi

  • High binding capacity, at least 40 mg/ml chromatography medium.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Note legali

HisTrap is a trademark of Cytiva

Pittogrammi

Environment

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

This page shows how to perform a purification of His-tagged membrane proteins.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Vedi tutto

Protocolli

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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