G4510
Glutathione−Agarose
lyophilized powder
Sinonimo/i:
Glutathione-Agarose Resin, GSH-agarose, S-linked glutathione agarose
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About This Item
Prodotti consigliati
Stato
lyophilized powder
Livello qualitativo
Classi chimiche degli analiti
proteins (GST)
Grado di funzionalizzazione
≥10 μmol per mL gel
tecniche
immunoprecipitation (IP): suitable
protein purification: suitable
Matrice
4% cross-linked beaded agarose
Attivazione matrice
epoxy
Gruppi immobilizzati alla matrice
sulfur
Braccio spaziatore
12 atoms (10 carbon)
Temperatura di conservazione
−20°C
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Descrizione generale
Glutathione S-transferase (GST) is a protein with a molecular weight of 26 KDa that naturally occurs in eukaryotic cells. It belongs to the family of Phase II detoxification enzymes. Glutathione-Agarose consists of glutathione attached through its sulfur to epoxy-activated 4% cross-linked beaded agarose, resulting in a 12-atom (10-carbon) spacer. It is provided as a lyophilized powder stabilized with lactose.
Applicazioni
Glutathione-Agarose has been used:
- to purify glutathione S-transferase (GST)-fused protein for generating polyclonal antibody M4P
- to incubate supernatant fractions for the purification of full-length p53 (aa 1-393) and its DNA-binding domain (aa 94-288)
- in affinity purification of the fusion protein
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.
Azioni biochim/fisiol
Glutathione S-transferases (GSTs), a superfamily of multifunctional detoxification isoenzymes, have a significant role in cellular signaling. GSTs are emerging as promising therapeutic targets due to the overexpression of specific isozymes in various tumors and their potential involvement in the development of other diseases such as neurodegenerative diseases, multiple sclerosis, and asthma.
Caratteristiche e vantaggi
- Offers rapid, mild, non-denaturing, and highly selective purification of glutathione-binding enzymes.
- Has a shelf life of two years if stored properly desiccated at -20 °C.
- Binding capacity: 5-10 mg glutathione S-transferase per mL resin
Quantità
One g powder swells to ≥10 ml of gel
Stato fisico
Lyophilized powder stabilized with lactose
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Contenuto correlato
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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