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Key Documents

G1767

Sigma-Aldrich

Glycogen from mussel, Mytilus genus

for DNA precipitations

Sinonimo/i:

DNA and RNA precipitation

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About This Item

Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
12352200
NACRES:
NA.52

Grado

for molecular biology

Livello qualitativo

Forma fisica

liquid

Confezionamento

vial of 1 mL

Concentrazione

19-22 mg/mL

Temperatura di conservazione

−20°C

InChI

1S/C24H42O21/c25-1-5-9(28)11(30)16(35)22(41-5)39-4-8-20(45-23-17(36)12(31)10(29)6(2-26)42-23)14(33)18(37)24(43-8)44-19-7(3-27)40-21(38)15(34)13(19)32/h5-38H,1-4H2/t5-,6-,7-,8-,9-,10-,11+,12+,13-,14-,15-,16-,17-,18-,19-,20-,21+,22+,23-,24-/m1/s1
BYSGBSNPRWKUQH-UJDJLXLFSA-N

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Descrizione generale

Glycogen is a branched polymer of glucose synthesized by animal cells for energy storage and release. It is constructed of predominantly α1→4 glycosidic bonds with branches created through α1→6 glycosidic bonds.
Glycogen is a branched polymer of glucose that coprecipitates nucleic acids during isolation and purification.

Applicazioni

Glycogen from Mytilus genus (Blue mussel) has been used


  • as a coprecipitant in the isolation of RNA from hepatocytes and yeast cells.
  • as a reference standard in calibration curve generation to quantify hepatic glycogen content.
  • as a carrier molecule for DNA and RNA, replacing tRNA or sonicated DNA
  • for the precipitation of DNA in the presence of ammonium acetate and ethanol or isopropanol
  • for recovery of tRNA, yeast RNA, sonicated DNA and oligonucleotides as short as 8 base pairs

Azioni biochim/fisiol

Glycogen levels in Mytilus genus shows variation during seasonal changes. It is a crucial stored energy reserve for Mussels and is utilized during gametogenesis. Glycogen levels dictate the reproductive capacity and development of mussels larva. Glycogen aids in the coprecipitation of nucleic acids during isolation and purification.

Caratteristiche e vantaggi

  • product provided is 1 mL total volume
  • precipitates even small amounts of DNA and RNA present in dilute solutions
  • does not interfere with OD 260/280 readings

Principio

Glycogen is an inert carrier that traps nucleic acids. It forms a precipitate with DNA/RNA while being insoluble in ethanol solution. Due to the manufacturing process, there is low risk of contaminating nucleic acids in the glycogen. This product is tested for DNase, RNase, and NICKase, but not nucleic acids.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

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Biomass composition of blue mussels, Mytilus edulis, is affected by living site and species of ingested microalgae
Pleissner D, et al.
ISRN zoology, 2012 (2012)
Tri-methylation of histone H3 lysine 4 facilitates gene expression in ageing cells
Cruz C, et al.
eLife, 7, e34081-e34081 (2018)
Regulation of gluconeogenesis by Kruppel-like factor 15
Gray S, et al.
Cell Metabolism, 5(4), 305-312 (2007)
Energy storage and reproduction in mussels, Mytilus galloprovincialis: the influence of diet quality
Fearman JA, et al.
Journal of shellfish research., 28(2), 305-312 (2009)
Tue Fryland et al.
Genome medicine, 8(1), 53-53 (2016-05-05)
The bromodomain containing 1 (BRD1) gene has been implicated with transcriptional regulation, brain development, and susceptibility to schizophrenia and bipolar disorder. To advance the understanding of BRD1 and its role in mental disorders, we characterized the protein and chromatin interactions

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