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F5803

Sigma-Aldrich

o-Dianisidine dihydrochloride

Suitable for use in glucose determination

Sinonimo/i:

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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About This Item

Formula empirica (notazione di Hill):
C14H16N2O2 · 2HCl
Numero CAS:
20325-40-0
Peso molecolare:
317.21
Beilstein:
3917996
Numero CE:
243-737-5
Numero MDL:
MFCD00012488
Codice UNSPSC:
12171500
ID PubChem:
24894894
NACRES:
NA.47

Livello qualitativo

200

Stato

crystalline

Intervallo di pH

6.95-7.05

Punto di fusione

268 °C

Solubilità

water: 2.5 mg/mL, colorless

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

2-8°C

Stringa SMILE

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H
UXTIAFYTYOEQHV-UHFFFAOYSA-N

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Descrizione generale

A preweighed vial containing 50 mg o-Dianisidine.

Specificità

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Applicazioni

o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies:
  • As substrate for the determination of serum ceruloplasmin from its oxidase activity.
  • Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method.
  • As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD).
  • Myeloperoxidase activity assay.

Attenzione

Probably carcinogenic.

Enzima

N° Catalogo
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Standard

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Descrizione
Determinazione del prezzo

Avvertenze

Danger

Indicazioni di pericolo

H302,H314,H350

Classi di pericolo

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificati d'analisi (COA)

Lot/Batch Number
SLCF9565
SLCB2275
SLBT8735
SLBN4014V
SLBK7700V

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Wijitar Dungchai et al.
Analytica chimica acta, 674(2), 227-233 (2010-08-04)
We report here the use of multiple indicators for a single analyte for paper-based microfluidic devices (microPAD) in an effort to improve the ability to visually discriminate between analyte concentrations. In existing microPADs, a single dye system is used for
Measurement of ceruloplasmin from its oxidase activity in serum by use of o-dianisidine dihydrochloride.
K H Schosinsky et al.
Clinical chemistry, 20(12), 1556-1563 (1974-12-01)
W Shi et al.
Journal of dairy science, 102(4), 3082-3096 (2019-02-11)
The objective of this study was to evaluate the effects of supplementing a Saccharomyces cerevisiae fermentation product (SCFP; NutriTek, Diamond V, Cedar Rapids, IA) during the periparturient period (d -28 ± 3 to 44 ± 3 relative to calving) on
Risheng Ye et al.
The Journal of clinical investigation, 128(3), 1178-1189 (2018-02-20)
The compensatory proliferation of insulin-producing β cells is critical to maintaining glucose homeostasis at the early stage of type 2 diabetes. Failure of β cells to proliferate results in hyperglycemia and insulin dependence in patients. To understand the effect of
L O Chow et al.
Journal of dairy science, 91(4), 1534-1543 (2008-03-20)
Objectives of the study were to evaluate the effect of planting date on in vitro neutral detergent fiber digestibility (IVFD) of whole-crop barley (Hordeum vulgare) and its effects on productivity of lactating dairy cows. Two cultivars of barley were planted
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Protocolli

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

Enzymatic Assay of Glucose Oxidase

Enzymatic Assay of Glucose Oxidase

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