F3133

Fibroblast Growth Factor from bovine pituitary

lyophilized, suitable for cell culture

• Sinonimo/i: FGF
• Numero CAS: 62031-54-3
• Numero MDL: MFCD00166724
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• product name: Fibroblast Growth Factor from bovine pituitary, suitable for cell culture
• Origine biologica: bovine pituitary glands
• Livello qualitativo: 200
• Forma fisica: lyophilized powder
• Potenza: 1-100 ng/mL
• PM: 16.4 kDa
• Confezionamento: pkg of 10 and 5X10 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• tecniche: cell culture | mammalian: suitable
• Impurezze: endotoxin, tested
• Colore: white to off-white
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.

Applicazioni

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.

Fibroblast Growth Factor from bovine pituitary has been used in in vitro testicular cell culture. It has also been used as a component of myogenic culture medium.

Azioni biochim/fisiol

Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis and organogenesis. It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells. Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival. FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.

Qualità

Although the major mitogenic component of this product is FGF2, the activity per unit weight of protein is less than that of purified FGF2.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg/ml BSA.

Nota sulla preparazione

Purified glands by a modification of the method of Gospodarowicz.

Risultati analitici

The biological activity is measured using a 3-day MTT assay.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)