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Key Documents

E7659

Sigma-Aldrich

Anti-EEA1 antibody, Mouse monoclonal

clone EEA1-N19, purified from hybridoma cell culture

Sinonimo/i:

Anti-MST105, Anti-MSTP105, Anti-ZFYVE2, Monoclonal Anti-Early endosomal antigen 1

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

EEA1-N19, monoclonal

Forma fisica

buffered aqueous solution

PM

antigen ~160 kDa

Reattività contro le specie

human, mouse, rat

Concentrazione

~1.0 mg/mL

tecniche

immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using whole extract of rat NRK cells

Isotipo

IgG2a

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... EEA1(8411)
mouse ... Eea1(216238)
rat ... Eea1(314764)

Descrizione generale

Monoclonal Anti-EEA1 (mouse IgG2a isotype) is derived from the hybridoma EEA1-N19 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human EEA1. Early Endosome Antigen 1 (EEA1) is a dimer. It comprises extensive coiled-coil regions, and at its C-terminus contains a cysteine-rich zinc-finger-like domain named FYVE domain.
The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins. It is a peripheral membrane protein associated with the cytoplasmic face of early endosomes. EEA1 is a autoantigen associated with subacute cutaneous systemic lupus erythematosus and is a coiled-coil protein localized to early endosomes and cytosol.

Applicazioni

Anti-EEA1 antibody, Mouse monoclonalhas been used in:
  • immunoblotting
  • immunoprecipitation
  • cell fractionation
  • indirect immunofluorescence
  • immunohistochemistry

Monoclonal Anti-EEA1 antibody produced in mouse is suitable for the following applications:
  • Immunoprecipitation
  • Indirect immunofluorescence
  • Western blotting at a concentration of 1-2μg/mL using whole extract of rat NRK cells

Azioni biochim/fisiol

Early Endosome Antigen 1 (EEA1) FYVE domain is involved in intracellular trafficking and is implicated in the specific localization of EEA1 to endosomes . Endosomal targeting of EEA1 also requires its binding to the active form of the small GTPase Rabaptin-5 (Rab5). The binding of EEA1 to phosphatidylinositol 3-phosphate (PtdIns 3P) and Rab5-GTP is essential for the localization and function of EEA1 in endocytic membrane fusion. Monoclonal Anti-EEA1 may be used as an early endosome marker.
Early Endosome Antigen 1 (EEA1) controls vesicle fusion during endocytosis. In neurons, it is involved in recycling of synaptic vesicles and neurotransmitter receptors. Patients with neurological deficits develop EEA1 autoantibodies. Mutation in EEA1 gene leads to susceptibility to diabetes in the Japanese population. Phosphatidylinositol 3-phosphate (PtdIns(3)P) is essential for the localization and function of EEA1.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Cellular functions of phosphatidylinositol 3-phosphate and FYVE domain proteins
GILLOOLY DJ, et al.
The Biochemical Journal, 355, 249-258 (2001)
Sequential roles for phosphatidylinositol 3-phosphate and Rab5 in tethering and fusion of early endosomes via their interaction with EEA1
Lawe DC, et al.
The Journal of Biological Chemistry, 277(10), 8611-8617 (2002)
Timely regulated sorting from early to late endosomes is required to maintain cerebellar long-term depression
Kim T, et al.
Nature Communications, 8(1), 401-401 (2017)
Biogenesis of the sorting endosome: the role of Rab5
Woodman PG
Traffic, 1(9), 695-701 (2000)
Aline Raquel Voltan et al.
Medical mycology, 51(7), 759-764 (2013-04-10)
Paracoccidioidomycosis (PCM) is a chronic granulomatous disease caused by the dimorphic fungus Paracoccidioides brasiliensis, endemic in Latin America. P. brasiliensis has been observed in epithelial cells in vivo and in vitro, as well as within the macrophages. The identification of

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