D8045
AccuTaq™ LA DNA Polymerase
High fidelity Taq enzyme, with 10X buffer & DMSO
Sinonimo/i:
High Fidelity DNA polymerase, High fidelity PCR, Long range PCR
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About This Item
Prodotti consigliati
Livello qualitativo
Forma fisica
liquid
impiego
sufficient for 125 reactions
sufficient for 1500 reactions
sufficient for 500 reactions
Caratteristiche
Long & Accurate PCR
dNTPs included: no
hotstart: no
Concentrazione
5 units/μL
tecniche
PCR: suitable
Colore
colorless
input
purified DNA
Compatibilità
suitable for PCR
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Categorie correlate
Descrizione generale
AccuTaq™ long and accurate (LA) DNA Polymerase is a combination of Taq DNA polymerase with a small amount of a polymerase with the 3′→5′ exonuclease activity necessary for proofreading. It enables the amplification of genomic targets larger than 20 kb. This blend increases the length of amplification products by using the proofreading polymerase to repair terminal misincorporations. This repair enables the polymerase to resume elongating the growing DNA strand. After the proofreading activity, the final products mostly have a blunt end.
Applicazioni
AccuTaq™ LA DNA Polymerase produces long DNA amplicons for:
- Mutation analysis
- Cloning genes
- DNA sequencing
- cDNA library generation
- Polymerase chain reaction (PCR)
- Direct PCR
Caratteristiche e vantaggi
- Increased fidelity, up to 6.5× that of Taq DNA polymerase
- Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA.
Confezionamento
The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates. A separate vial of DMSO is included. Addition of DMSO in the reaction at a final concentration of 1-4% may increase yield and improve reliability of the system with some complex PCR targets.
Altre note
AccuTaq LA DNA polymerase is an optimized blend of Sigma′s high quality Taq DNA polymerase and a small amount of an additional polymerase that exhibits 3′→5′ exonuclease or proofreading activity. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate.
Store AccuTaq LA DNA Polymerase and the 10x Buffer at –20 °C. Store DMSO at room temperature. Melting frozen DMSO at ~30 °C will not affect performance.
Definizione di unità
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74°C.
Note legali
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC
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Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3 - Resp. Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
188.6 °F
Punto d’infiammabilità (°C)
87 °C
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Articoli
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
Protocolli
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
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