CRISPR11V

CRISPR-Lenti Human EMX1 Positive Control Transduction Particles

• Codice UNSPSC: 12352200
• NACRES: NA.51

Proprietà

• Forma fisica: liquid
• Livello qualitativo: 200
• Confezionamento: vial of 200 μL
• Concentrazione: 1x10⁶ VP/ml (via p24 assay)
• applicazioni: CRISPR
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

The Sigma CRISPR-LENTI HUMAN EMX1 POSITIVE CONTROL TRANSDUCTION PARTICLES are a critical positive control to monitor transduction efficiency for lenti CRISPRs. This control is produced from the sequence-verified CRISPR lentiviral plasmid targeting human EMX1. EMX1 is one of the members of the EMX homeobox family, which are expressed in the developing cerebrum and involved in specification of positional identity and neural cell proliferation. This targets a validated EMX1 CRISPR site, which serves as an experimental control for Sigma lenti CRISPRs. The Sigma lenti CRISPR positive control is a one-component system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA plasmid targeting the human EMX1 gene, with both Puromycin and GFP co-expressed with Cas9.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.

Applicazioni

Functional Genomics/Screening/Target Validation

• Positive control, CRISPR lentivirus, viral format, for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit sigma.com/crisprs.

Caratteristiche e vantaggi

Serves as a positive experimental control for the CRISPR editing workflow using lenti CRISPRs (virus format). Allows for validation of your system with the lenti CRISPR/Cas9 system. A positive result in a mis-match detection assay will indicate validation of your system.

Stato fisico

200 μl of 10⁶ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

Note legali

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable